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| Effects of Different Concentrations of EMS on Mutagenic Non-heading Chinese Cabbage |
| ZHOU Yucai1，2，XIAO Dong1，LI Ying1，2，HE Ying1，2，HOU Xilin1，2* |
| 1National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（East China），Ministry of Agriculture and Rural Affairs of China，Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops，Ministry of Education of China，Nanjing Agricultural University，Nanjing 210095，Jiangsu，China；2Nanjing Suman Plasma Engineering Research Institute Co.，Ltd.，Nanjing 211162，Jiangsu，China |
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Abstract In order to create new germplasm and discover new genes，we explored the mutagenic effect
of different concentrations of ethyl methane sulfonate（EMS）on non-heading Chinese cabbage．Four varieties of non-heading Chinese cabbage（HTC，XQC，JP241 and XWZ）were subjected to seed mutation treatment with EMS at concentrations of 0.4%，0.6%，0.8%，and seed germination and the variation of agronomic traits in the field were investigated．The results showed that the seed germination potential，germination rate and relative seedling establishment rate of the four varieties of non-heading Chinese cabbage gradually decreased with the increase of EMS concentration，and the differences between treatments were significant．EMS mutagenesis cause variations in the traits of leaf color，leaf shape，plant type and flowering organs of non-heading Chinese cabbage．The EMS-induced LD50 of different varieties of non-heading Chinese cabbage were different，and the types and frequencies of variation were also different．The LD50 of HTC was 0.8%，and the relative frequency of variation was 23.83%；the LD50 of XQC was 0.8%，and the relative frequency of variation was 17.61%；the LD50 of JP241 was 0.6%，and the relative frequency of variation was 38.45%；the LD50 of XWZ was 0.6%，and the relative variation frequency of 16.69%．As the concentration of EMS increased，the later the flowering time of the first flower of mutant non-heading Chinese cabbage，the fewer the number of rosette leaves，and the higher the male sterility rate．
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Received: 11 November 2023
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